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. 2022 Apr 11;8:22. doi: 10.1038/s41522-022-00288-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Venn analysis of bacterial communities at the OTU level among rearing water, biofloc, and gut in control and sucrose addition culture systems. b The relative abundances of bacterial taxa at genus level shared by rearing water, biofloc, and gut in each culture system. c The percentage contributions of bacterial sources for biofloc and gut samples in each culture system based on the SourceTracker analysis. d The numbers and relative abundances of core and specific OTUs in each culture system. CK: without sucrose addition; CN10: the C/N ratio of about 10:1 obtained by sucrose addition; CN15: the C/N ratio of about 15:1 obtained by sucrose addition. Each sample had six replicates (n = 6), except the water samples (n = 4) and biofloc samples (n = 2) in control group. Error bars show standard error in (d). Different asterisks indicate a significant difference at *p < 0.05, **p < 0.01, and ***p < 0.001 in (c) and (d) based on Student’s t test.