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. 2022 Apr 11;8:22. doi: 10.1038/s41522-022-00288-x

Table 1.

ANOSIM and PERMANOVA analysis between different groups.

Group Process ANOSIM PERMANOVA
R P_adj_BH F value Variation (R2) P_adj_BH
CK Water vs Biofloc 0.93 0.07 4.22 0.51 0.067
Water vs Gut 0.80 0.007 6.95 0.47 0.008
Biofloc vs Gut 0.47 0.154 2.33 0.28 0.045
CN10 Water vs Biofloc 0.37 0.007 2.72 0.21 0.008
Water vs Gut 0.69 0.007 3.75 0.27 0.008
Biofloc vs Gut 0.65 0.007 3.94 0.28 0.008
CN15 Water vs Biofloc 0.47 0.008 2.98 0.23 0.008
Water vs Gut 0.73 0.007 4.87 0.33 0.013
Biofloc vs Gut 0.66 0.007 4.18 0.29 0.008
Water CK vs CN10 0.66 0.018 4.87 0.38 0.011
CK vs CN15 0.98 0.008 7.67 0.49 0009
CN10 vs CN15 0.40 0.007 2.24 0.18 0.011
Biofloc CK vs CN10 0.90 0.036 3.80 0.39 0.039
CK vs CN15 0.94 0.031 4.34 0.42 0.040
CN10 vs CN15 0.66 0.007 3.63 0.27 0.008
Gut CK vs CN10 0.61 0.007 3.90 0.28 0.008
CK vs CN15 0.88 0.007 8.93 0.47 0.008
CN10 vs CN15 0.71 0.007 4.45 0.31 0.008

ANOSIM and PERMANOVA analysis were based on Bray-Curtis dissimilarity for the comparison of bacterial community composition in the water, biofloc, and gut samples between control and treatment (permutation = 999). Each sample had six replicates (n = 6), except the water samples (n = 4) and biofloc samples (n = 2) in control group. P values adjusted by using the Benjamini–Hochberg algorithm <0.05 indicate significant differences.