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. 2022 Apr 11;12:6066. doi: 10.1038/s41598-022-09778-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relationship between PACE4-altCT and ERG status in prostate cancer cells. (A) Western blot analysis on LNCaP cell lysates following transfections with the indicated vectors. Each lane represent an individual transfection. ERG is shown with distinct exposures. These blots were cropped to emphasize the intended band. Uncropped original blots are provided as in Supplementary Material. (B) PACE4 densitometry analysis using actin as a reference. Data are mean ± SEM. (C) PACE4 splicing indices determined by RT-qPCR in transfected LNCaP cells. Data are mean ± SEM (n = 3).