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. 2022 Apr 11;12:5181. doi: 10.1038/s41598-022-09017-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MAC induces IL-8 production by neutrophil. (A) Proteome profile of chemokines and cytokines in neutrophils treated with mycobacteria. Representative results are shown. (B) IL-8 production by neutrophils treated with mycobacteria. (C–E) Effects of PAD4 inhibitor on cytokine production by neutrophils treated with mycobacteria. The production of IL-8 (B,C), IL-1β (D) and TNF-α (E) by neutrophils was measured by ELISA. Data are means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test.