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. 2022 Apr 11;5:344. doi: 10.1038/s42003-022-03308-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Gene delivery capacity of ternary transformation system. Gene delivery was represented by the number of transgenic cells exhibiting YFP fluorescence on the surface of sorghum embryos. d–f Tissue culture response represented by callus proliferation after two weeks of Agrobacterium infection on the multi-purpose medium without selection. g–i YFP images of early-stage somatic embryo formation (indicated by the arrows) induced by morphogenic gene expression on the surface of immature scutella. j–l Shoot regeneration after four-week of maturation. m–o The pleiotropic impacts of morphogenic gene expression on single-copy QEs. a, d, g, j and m Tx430. b, e, h, k, and n Tx623. c, f, i, l and o Tx2752.