Table 1.
Transformation and event quality efficiencies of different sorghum transformation systems.
| Transformation system | Construct | Selectable marker | Genotype | # of embryos infected | # of T0 plants (eff.)a | # of T0 QE (freq.)b | # of Marker-free T0 plants (eff.)c | # of T0 escape plants (freq.)d | # of Co-transformation in T0 plants (freq.)e |
|---|---|---|---|---|---|---|---|---|---|
| Conventional | pPHP86655 | NPTII | Tx430 | 472 | 99 (21.9%) | 58 (58.5%) | 0 | ||
| pPHP87980 | NPTII | Macia | 973 | 7 (0.7%) | NA | NA | |||
| Integrated morphogenic gene-enabled | pPHP79066 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Tx430 | 250 | 97 (38.8%) | 47 (48.5%) | 0 | ||
| pPHP79066 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Tx623 | 246 | 16 (6.5%) | NA | NA | |||
| pPHP79066 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Tx2752 | 200 | 19 (9.5%) | NA | NA | |||
| Morphogenic gene-enabled excision-induced selection-activation | pPHP81814 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Tx430 | 248 | 173 (69.7%) | 66 (38.2%) | 0 | ||
| pPHP81814 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Macia | 249 | 50 (20.0%) | NA | NA | |||
| pPHP81814 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Tegemeo | 210 | 36 (17.1%) | NA | NA | |||
| pPHP81814 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Malisor 84-7 | 230 | 50 (21.7%) | NA | NA | |||
| pPHP86482 (Axig1pro:Wus2/Pltppro:Bbm) | Hra | Tx430 | 241 | 147 (60.9%) | 69 (46.9%) | 0 | |||
| Integrated Wus2-enabled | pPHP96564 (Pltppro:Wus2) | NPTII | Tx430 | 378 | 222 (58.7%) | 117 (52.7%) | 0 | ||
| pPHP96564 (Pltppro:Wus2) | NPTII | Tx623 | 247 | 39 (15.8%) | 19 (48.7%) | 0 | |||
| Wus2/CRE-enabled marker-free | pPHP94632 (Pltppro:Wus2) | NPTII | Tx430 | 185 | 54 (29.2%) | 17 (31.4%) | 32 (59.2%) | 2 (3.7%) | |
| pPHP94292 (Pltppro:Wus2) | NPTII | Tx430 | 169 | 54 (31.9%) | 19 (35.2%) | 33 (61.1%) | 0 | ||
| Non-integrated Wus2-enabled altruistic | (90%) pPHP86655 + (10%) pPHP87078 (3xENH:Pltppro:Wus2/Sb-Ubipro:Zs-GREEN1) | NPTII | Tx430 | 370 | 135 (36.5%) | 76 (56.3%) | 0 | 6 (4.4%) | |
| (90%) pPHP87980 + (10%) pPHP88158 (3xENH:Pltppro:Wus2/NOSpro:Crc) | NPTII | Macia | 1072 | 162 (15.1%) | 95 (58.6%) | 0 | 7 (4.3%) |
NA, not available.
aThe number in the parentheses represents the transformation efficiency. The transformation efficiency was calculated as the number of regenerated shoots recovered per 100 embryos infected.
bFor conventional and morphogenic gene-enabled transformations, the transgenic plant carrying a single copy of the intact T-DNA integration without vector backbone was defined as ‘quality event (QE)’. For morphogenic gene-enabled excision-induced selection-activation transformation, the transgenic plant carrying a single copy of the intact T-DNA integration without vector backbone and Wus2/Bbm/moCRE/GREEN1 cassette was defined as ‘quality event (QE)’. For Wus2/moCRE-enabled marker-free transformation, the transgenic plants carrying a single copy of the intact T-DNA integration without vector backbone and Wus2/moCRE/NPTII cassette was defined as ‘quality event (QE)’. For altruistic transformation, the transgenic plant carrying a single copy of the intact T-DNA integration without vector backbone and altruistic T-DNA co-integration was defined as ‘quality event (QE)’. The number in the parentheses represents the percentage of QE.
cThe number in the parentheses represents the percentage of Wus2/moCRE/NPTII cassette excision efficiency or marker-free efficiency.
dThe number in the parentheses represents the percentage of non-transgenic escapes.
eThe number in the parentheses represents the percentage of co-transformation.