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. 2022 Apr 11;5:343. doi: 10.1038/s42003-022-03250-x

Fig. 4. LEVER RNA sequesters EZH2 and blocks histone H3K27 methylation at the LEVER locus in cis.

Fig. 4

a, b EZH2 (a) or H3K27me3 (b) chromatin occupation at selected regions within the LEVER locus. MYT1 and CCDC26 serve as ChIP positive and negative controls, respectively. LVR-P primer pair is designed at the LEVER promoter region. Data from n = 3 biological replicates are shown. Error bars represent SEM. Statistical difference was calculated using Welch’s one-tailed t-test. ns, not significant. c H3K27me3, H3K27me2, and H3K27ac ChIP-seq profiling at LEVER and its neighboring locus in dox-inducible non-targeting control (NTC) or LEVER knock-down (LEVER KD) K562 cells treated with dox for 28 days. d H3K27me3 ChIP-qPCR of EZH2-KO and rescued cells as in Fig. 3a, b at LVR-4, LVR-3, and LVR-2 regions.