FIGURE 1.

Dual antiplatelet therapy of aspirin + cangrelor effectively prevents thrombosis in the absence of tissue factor. (A) Whole blood was treated with aspirin (500 μM) + cangrelor (10 μM) (A + C) to provide DAPT, or their vehicles as control, before perfusion over collagen in a bifurcated microfluidic model of occlusive thrombosis. Platelets (and leukocytes) were labeled with DiOC₆. Fibrin accumulation was monitored using Alexa Fluor‐546–labeled fibrinogen. Platelet (and leukocyte) and fibrin accumulation on collagen were visualized using confocal microscopy over a 24‐minute time course. The images are representative of 6 donors. Mean platelet (green) and fibrin (pink) accumulation ± standard deviation (SD), and mean flow rate are shown in the panels to the right (SD is shaded are for flow rate; n = 6). (B) The platelet and fibrin integrated density values at 24 minutes (**P < .05; no statistically significant difference in fibrin formation was found between control and treated experiments). (C) Time to occlude for each experiment. In control experiments, 1 of 6 channels did not occlude as is marked >24. In A + C‐treated experiments, occlusion was prevented in 5 of 6 channels. DMSO, dimethyl sulfoxide