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. 2022 Mar 29;9:821404. doi: 10.3389/fnut.2022.821404

TABLE 1.

GenBank accession numbers, sequences of forward and reverse primers, and fragment sizes used for real-time PCR.

Target GenBank number Primer sequence Size, bp
TNF NM_013693.3 F:5′ TTGTCTACTCCCAGGTTCTCT3′ 107
R: 5′ GAGGTTGACTTTCTCCTGGTATG3′
IL-6 NM_031168.2 F:5′ CTTCCATCCAGTTGCCTTCT3′ 134
R:5′ CTCCGACTTGTGAAGTGGTATAG3′
IL-1β NM_008361.4 F:5′ CCACCTCAATGGACAGAATATCA3′ 96
R:5′ CCCAAGGCCACAGGTATTT3′
GAPDH NM_001289726.1 F:5′ AACAGCAACTCCCACTCTTC3′ 111
R:5′ CCTGTTGCTGTAGCCGTATT3′