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. 2022 Mar 29;9:819753. doi: 10.3389/fnut.2022.819753

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Copyright © 2022 Reddy, Stander, Stafford and Makunga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Feature-based molecular network of Sceletium populations showing structural trends. Annotated nodes with pentagon boarders represent putatively identified metabolites (Table 2). Red outlines represent the mesembrine alkaloids, blue outlines represent joubertiamine alkaloids and orange outlines represent Sceletium A4 alkaloid class. Nodes are colored by retention time (RT) with their size representing the abundance of the metabolite. The distance between nodes indicates structural similarity based on fragmentation patterns from tandem mass spectrometry data. (B) Feature-based molecular network of Sceletium populations showing phytochemical distribution trends across populations. Red: S. tortuosum (De Rust); Blue: S. strictum (Anysberg); Green: S. expansum (Calitzdorp) and Purple: S. rigidum (Prince Albert).