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. 2022 Mar 29;13:840763. doi: 10.3389/fphys.2022.840763

FIGURE 1.

FIGURE 1

Only cognate agonists of CLR-RAMP complexes recruit β-arrestins and induce internalisation. HEK293T cells expressing CLR-RAMP complexes were assayed for cAMP accumulation (A), β-arrestin1 (B), β-arrestin2 (C) recruitment, and internalisation (D) at CLR in complex with RAMP1, RAMP2, and RAMP3. (E) Responses to 10 μM or 1 μM CGRP, AM, and AM2 at cAMP, β-arrestin1, β-arrestin2, and internalisation. Statistical significance between RAMPs for each peptide was determined, at p < 0.05, through One-Way ANOVA with Dunnett’s post-test (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). Data are shown as mean with error bars indicating the SEM of n repeats where n ranges between 3 and 5 duplicates.