FIGURE 2.

CLR-RAMP1 in the only complex to undergo appreciable internalisation and trafficking, leading towards degradation. Schematic (created with BioRender.com) showing the internalisation and subcellular trafficking of CLR in complex with a RAMP, identifying the different membrane GTPases used (A). Change in colocalisation of CLR over 60 min stimulation with each peptide as determined in HEK293T cells, for RIT, Rab5a, Rab7, and Rab11 when in complex with RAMP1, RAMP2, and RAMP3 (B). Data are shown as mean with error bars indicating the SEM of n repeats where n = 3 duplicates.