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. 2022 Mar 29;9:819921. doi: 10.3389/fvets.2022.819921

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Copyright © 2022 Zhu, Zhao, Zhao, Liu, Xia, Zhang, Wang, Zhang, Xu, Chen, Jiang, Wu, Wu, Zhang, Bai, Hu, Fotina, Wang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The mechanism of action of MPX activity on S. aureus. (A) The BCA kit was used to detect the results of total protein content in bacterial supernatant. (B) Uptake of NPN by S. aureus treated with MPX. (C) DiSC3-5 was used to detect changes in the membrane potential of S. aureus after MPX treatment. (D) Fluorescence microscopy observation of PI staining after MPX treatment. (E) Visual observation of the mechanism of MPX on S. aureus ATCC 25923 by transmission electron microscopy. Error bars indicate the mean ± SEM, n = 3. Statistical significance was defined as **p < 0.01.