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. 2022 Mar 29;9:819921. doi: 10.3389/fvets.2022.819921

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Copyright © 2022 Zhu, Zhao, Zhao, Liu, Xia, Zhang, Wang, Zhang, Xu, Chen, Jiang, Wu, Wu, Zhang, Bai, Hu, Fotina, Wang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MPX inhibits biofilm formation. (A) The results of crystal violet staining of biofilms after different concentrations of MPX were applied to S. aureus ATCC 25923. (B) OD₅₇₀ measurement after crystal violet was dissolved in 70% ethanol. (C) After exposure of S. aureus ATCC 25923 to different concentrations of MPX, scanning electron microscopy was used to observe the biofilm. (D) The results of staining S. aureus with SYTO 9/propidium iodide. Error bars indicate the mean ± SEM, n = 3. Statistical significance was defined as follows: **p < 0.01; ***P < 0.001; ns P > 0.05.