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. 2022 Mar 29;9:818302. doi: 10.3389/fmolb.2022.818302

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Copyright © 2022 Lim, Isshiki, Iki, Kawaguchi and Kai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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piRNAs mapping to canonical piRNA clusters and transposons are downregulated in kots mutants. (A) Log₂ fold change (mutant/control) of piRNAs mapping to 8 major clusters in mutant and control gonadal samples. (B) Downregulation of cluster-mapping piRNAs is more severe in testes than that in ovaries (t-test, p-value 0.013). (C) Boxplot showing Z₁₀ score of piRNAs mapping to clusters in ovaries and testes (t-test, p-values 0.005 and 0.189, respectively). (D) Log₂ fold change of top 20 most abundant TE-mapping piRNAs. (E) Comparison of the top 20 most abundant TE-mapping piRNAs in ovaries and testes (t-test, p-value 0.012). (F) Log₂ fold change in abundance of piRNAs mapped to TEs enriched in spermatogonia and spermatocytes.