TABLE 3.
Resistance-related amino acid changes with respect to the HIV-1 HXB2 sequence detected by sequencing of the RT genes of strains harboring the Q151M or T69S-SS mutation
| Patient identification-sample codea | Amino acid change in the following codonb:
|
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M41 | A62 | D67 | S68 | T69 | K70 | V75 | F77 | A98 | K103 | Y115 | F116 | Q151 | V179 | Y181 | M184 | L210 | T215 | K219 | |
| IT1-31.8 | N | R | M | Q | |||||||||||||||
| IT1-31.8R | N | R | M | Q | |||||||||||||||
| IT2-31.15 | V/I | L | Y | M | |||||||||||||||
| IT2-31.15R | L | Y | M | ||||||||||||||||
| SP1-9612024 | I | L | Y | Q/Mc | |||||||||||||||
| SP1-9612024R | I | L | Y | Q/M | |||||||||||||||
| SP2-9502063 | G | Sd | M | C | |||||||||||||||
| SW1-10671 | V | G | I | L | Sd | Y | M | Id | |||||||||||
| SW1-10671R | V | G | I | L | Sd | Y | M | Id | |||||||||||
| SW2-11546 | V | N | G | Gd | I | L | F | M | V | Y/Fe | Q | ||||||||
| SW2-11546R | N | G | Gd | L | R | M | W | Y | Q | ||||||||||
| SW3-10125 | L | V | S-SS | I | W | Y | |||||||||||||
| SW3-10125R | L | V | S-SS | V/Ad | I | W | Y | ||||||||||||
| CHG-4815 | S-SS | R | Y | ||||||||||||||||
The strains with suffix R refer to the recombinant virus.
All amino acid changes related to resistance are given. Slashes denote mixtures. A, alanine; C, cysteine; D, aspartic acid; F, phenylalanine; G, glycine; I, isoleucine; K, lysine; L, leucine; M, methionine; N, asparagine; Q, glutamine; R, arginine; S, serine; T, threonine; V, valine; W, tryptophan; Y, tyrosine.
The Q/M mixture was detected by ARMS-151 and LiPA-151; by sequencing only Q151M was detected.
Polymorphisms other than the resistance-related changes at positions associated with resistance.
T215Y/F was detected by LiPA HIV-1 RT; T215F was scored by sequencing.