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. 2022 Mar 29;13:870861. doi: 10.3389/fphar.2022.870861

FIGURE 7.

FIGURE 7

R2-Pep limits OGD-induced neuronal death and is more effective than baclofen. A, OGD-stressed cultures were treated with R2-Pep or Crtl-Pep immediately (0 h), 2, 4, 6 or 8 h after the stress. After 24 h the cultures were immunostained for neurons (NeuN, green) and total cells (DAPI, blue). Top: scheme of experimental design. Middle: representative images (scale bar: 100 μm). Bottom: Quantification of surviving neurons. The value of the untreated cultures served as control. The data represent the mean ± S.E.M. of 20 frames (fields of view) for each experimental condition derived from 2 independent experiments. ns: p > 0.05; *, p < 0.05; ***, p < 0.001; ****, p < 0.0001; Brown-Forsythe/Welch one-way ANOVA followed by Dunnett’s T3 multiple comparison test. B, OGD-stressed cultures were treated with baclofen immediately (0 h), 2 h or 4 h after the stress. After 24 h the cultures were immunostained for neurons (NeuN, green) and total cells (DAPI, blue). Top: representative images (scale bar: 100 μm). Bottom: Quantification of surviving neurons. The value of the untreated cultures served as control. The data represent the mean ± S.E.M. of 40–45 frames for each experimental condition derived from 3 independent experiments. ****, p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons test.