Table 3. Impacts of concentrations of HCl on extraction efficiencies and matrix effects of the three analytes.
| Concentration
(ng/mL) |
0.01 mol/L HCl | 0.05 mol/L HCl | 0.1 mol/L HCl | ||||||
| Extraction
efficiency (%) |
Matrix
effect (%) |
Extraction
efficiency (%) |
Matrix
effect (%) |
Extraction
efficiency (%) |
Matrix
effect (%) |
||||
| All the data were presented as mean±SD. 2-Oxo-CLP: 2-oxo-clopidogrel; CAMD: clopidogrel active metabolite derivative. | |||||||||
| Clopidogrel
|
0.1 | 170.9±8.9 | 364.0±10.2 | 107.4±6.2 | 75.0±2.8 | 194.4±9.8 | 52.9±2.9 | ||
| 20 | 147.5±6.1 | 107.4±4.5 | 116.5±2.2 | 94.1±10.0 | 155.7±7.4 | 52.1±4.0 | |||
| 40 | 138.8±5.4 | 88.2±4.3 | 118.7±8.7 | 71.9±5.9 | 134.0±6.1 | 49.0±3.1 | |||
| 2-Oxo-CLP
|
1 | 96.5±6.4 | 72.1±4.3 | 92.5±3.9 | 63.7±3.4 | 115.1±9.5 | 40.5±3.6 | ||
| 20 | 103.2±3.4 | 61.3±4.0 | 117.0±3.5 | 71.6±5.0 | 148.3±6.5 | 38.2±3.1 | |||
| 40 | 103.9±3.2 | 64.5±4.3 | 110.9±7.8 | 63.7±4.6 | 133.7±3.5 | 39.5±2.0 | |||
| CAMD | 1 | 32.1±2.3 | 84.0±2.5 | 96.7±3.2 | 70.8 ±5.3 | 93.4±13.3 | 52.2±6.3 | ||
| 40 | 34.8±1.6 | 80.7±3.8 | 123.4±3.9 | 53.0±5.8 | 171.6±5.3 | 39.7±2.1 | |||
| 80 | 34.0±2.6 | 88.4±2.3 | 120.2±7.0 | 59.3±5.0 | 167.9±5.1 | 53.8±4.6 | |||