TABLE 1.

Primer descriptions and MgCl₂ concentrations used in this study

PCR primer pair	Nucleotide sequence		Product size (bp)	Optimum MgCl2 concn (mM)	Base start	Base end
	5′	3′
23S rRNA primers
23S 5′	5′-GGTTAAGTTAATAAGGGCGC-3′	5′-TTTCGACTACGGATCTTAGC-3′	1,011	4	1	1011
23S center	5′-CTGTTTGGGTGAGGGGTCC-3′	5′-ACCTGCATCTTCACAGGTAC-3′	1,060	1	900	2041
23S 3′	5′-CGGCGGCCGTAACTATAACG-3′	5′-TTGGATAAGTCCTCGAGCTATTAG-3′	1,003	1	1899	2902
Unique 23S primers
DS_18	5′-CGGCGGCCGTAACTATAACG-3′	5′-GCCAGCTGAGCTACACCGCC-3′	1,900	2	1899	+897a
DS_23	5′-CGGCGGCCGTAACTATAACG-3′	5′-TACACACTCACATATCTCTG-3′	1,200	2	1899	+197a
DS_30	5′-CGGCGGCCGTAACTATAACG-3′	5′-TTTTACCACTAAACTACACC-3′	1,300	2	1899	+297a
DS_91	5′-CGGCGGCCGTAACTATAACG-3′	5′-TACCAACTGAGCTATGGCGG-3′	1,900	3	1899	+897a
Ribosomal protein primers
L4	5′-AAATCAGCAGTTAAAGCTGG-3′	5′-GAGCTTTCAGTGATGACAGG-3′	720	2	−50b	670b
L22	5′-GCAGACGACAAGAAAACACG-3′	5′-ATTGGATGTACTTTTTGACC-3′	420	2	−41b	380b
Erythromycin resistance primers
mph(C)	5′-GGGAAATTGAACACAAACC-3′	5′-AATTCATCTGATACRCCATAAG-3′c	500	3	1b	520b
erm(TR)	5′-ACAGAAAAACCCGAAAAATACG-3′	5′-TTGGATAATTTATCAAGATCAG-3′	679	1	5b	731b

^aBase relative to 3′ end of 23S rRNA. 

^bBase relative to ATG. 

^cR = A + G.