Fig. 2. AtGRF5 facilitates the CRISPR/Cas9-mediated genome editing of ClPDS. (a) Schematic diagrams of the genome-editing vector pZHW512. (b) Summary of the ClPDS genome-editing results. D indicates deletion. (c) Summary of the mutation types at two target sites in the ClPDS gene. The PAM sequences for the two target sites are highlighted in red. (d) Results from Sanger sequencing of the two target sites in the wild type (WT) and editing line #9. (e) Phenotype of the genome-edited ClPDS mutant plant. Scale bar=2 cm. AtGRF5: Arabidopsis thaliana growth-regulating factor 5; CRISPR: clustered regularly interspaced short palindromic repeats; Cas9: CRISPR-associated protein 9; ClPDS: Citrullus lanatus phytoene desaturase; PAM: protospacer adjacent motif. RB: right border; LB: left border.