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. 2022 Mar 29;13:811728. doi: 10.3389/fpls.2022.811728

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Copyright © 2022 Zhang, Pritchard, Mieog, Byrne, Colgrave, Wang and Ral.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of TaAMY1 overexpression on grain composition and starch morphology during development. TaAMY1 protein content expressed by optical density (A) and total α-amylase activity expressed in Ceralpha Units (CU) of per gramme of tissue (B), and total soluble sugar expressed in mg.g tissue^{– 1} (C) during grain development. Scanning electron micrographs (SEM) of representative grain cross sections from Bx17A1OE4.1 (D–H) and Fielder (wild type, WT) (I–M) during key grain development stage. White arrows indicated starch degraded by TaAMY1.