FIG. 2.

Restriction endonuclease map of frxA and rdxA clones from the lambda phage library constructed with genomic DNAs from Mtz-sensitive and -resistant H. pylori strains. The phage clones that carried an frxA gene or an rdxA gene were screened by plaque hybridization. Restriction fragments that contained an frxA or an rdxA gene were identified by Southern hybridization. The restriction fragments that contained the frxA or rdxA gene were inserted into pBluescript SK(+) digested with the same or appropriate restriction enzymes. pGH170 and pGH121 were cloned from the genomic DNA of H. pylori 2600 (Mtz MIC, 2 μg/ml), pGH175 and pGH179 were cloned from the genomic DNA of H. pylori ATCC 700392 (Mtz MIC, 8 μg/ml), pGH174 and pGH101 were cloned from the genomic DNA of H. pylori 6013 (Mtz MIC, 32 μg/ml), pGH178 and pGH160 were cloned from the genomic DNA of H. pylori 1857 (Mtz MIC, 128 μg/ml), and pGH180 and pGH68 were cloned from the genomic DNA of H. pylori 1700 (Mtz MIC, 256 μg/ml). E, EcoRI; E47, Eco47III; H, HindIII; N, NsiI; S, SphI; X, XbaI.