Enriched target genes of herbs in the hot and cold groups. (a) Enrichment analysis comparing the target genes of the HG with those of the CG and NHG (left) as well as comparing the target genes of the CG with those of the HG and NCG (right). The padj values were obtained by Benjamini–Hochberg multiple testing correction after hypergeometric tests. The hot- and cold-specific target genes are denoted with the gray boxes. * padj < 10−2, ** padj < 10−3, *** padj < 10−4, **** padj < 10−5. (b) Normalized ratios of the top five hot- or cold-specific target genes of the herbs of the CG, CGE, EG, HE, and HG, calculated as z-scores. (c) Pathway enrichment analysis of the target genes of the HG (upper panel) and CG (lower panel) using g:Profiler with the GO BP gene set. The top 10 functional characteristics were plotted, with padj < 0.05.