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. 2022 Mar 29;13:859680. doi: 10.3389/fmicb.2022.859680

FIGURE 1.

FIGURE 1

Leptospira PF07598 gene family members, represented by LA3490 here, are predicted with high confidence to have two tandemly repeated, N-terminal ricin B-like (RBL) lectin domains. (A) Visualization of an AlphaFold 3D-generated model of full-length LA3490 (Callaway, 2020; Jumper et al., 2020; Senior et al., 2020) showing four globular domains N-terminal to C-terminal (blue to red color) residues visualized in PyMOL 2.4.0 https://pymol.org/2/. Phyre2 (Protein Fold Prediction Server; http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index) had first predicted, with high (> 94%) confidence, that LA3490, as well as all other virulence-modifying (VM) proteins encoded by the PF07598 gene family, contains N-terminal β-trefoil folds identified as ricin B domains. (B) Ricin B domain (PBD; 2AAI-B, 7 aa to 129 aa). (C) Superimposition of 2AAI-B and N-terminal region of LA3490 (i.e., amino acid positions 40–150) was performed using PyMOL (TM) 2.4.0 showing structural conservation of RBL1 and the B chain of ricin (RMSD = 1.796 Å).