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. 2022 Mar 30;11(7):934. doi: 10.3390/plants11070934

Figure 4.

Figure 4

Activation of GR proteolytic degradation by leaves fractions. (A) Western blot analysis of GR was performed using commercially provided antibodies to evaluate GR and β-actin protein levels in HEK293 cell extracts pre-treated with 5 μM MG-132 for 1 h, and subsequently treated with vehicle or 50 μg/mL leaves fractions or 10 nΜ DEX, for 24 h in hormone depleted medium. (B) Quantification of the results. GR protein levels were normalized against the respective β-actin protein levels. Data are expressed as the mean ± SD, (n = 3), *** p < 0.001, compared to control vehicle-treated cells. ### p < 0.001, # p < 0.05 compared to the respective MG-132-treated cells.