Figure 8.

Induction of apoptosis by medium-polar (A,C) and polar (B,D) leaves fractions of South (A,B) and South versus North (C,D) Chios mastiha tree. Western blot analysis of β-actin, GAPDH, procaspase-3, procaspase-9 and Bcl-2 protein levels in cell extracts from HEK293 cells treated with 20 to 100 μg/mL of leaves fractions and/or 10 nM DEX, for 48 (C,D) and 72 h (A,B) in hormone-depleted medium was performed using commercially provided antibodies. β-actin or GAPDH protein levels were assessed for the normalization of the results.