Identification of expressed genes from L220, PH4CV, and their near-isogenic lines NIL220-3 and NIL220-25. (A) The number of expressed genes identified from different genotypes that germinated at 10 °C for 15 days and 25 °C for 3 days, and ungerminated dry embryo. Data were the average of two replications. (B) Heatmap clustering of the expressed genes. Pink, green, and blue columns presented vertically at the right side of the heatmap represent samples collected from dry embryo, seedlings germinated at 10 °C for 15 days, and seedlings germinated at 25 °C for 3 days, respectively. Red and blue represent high and low abundance, respectively, according to the normalized FPKM. The numbers 1 and 2 after the genotype represent replicates 1 and 2, respectively.