Figure 3.

Sorghum bran extracts reduced intracellular lipid accumulation in differentiated 3T3-L1 cells. (A) Experimental design. (B) 3T3-L1 cells were differentiated with the DMI cocktail for 2 days and treated with maintenance media containing different concentrations of sorghum extracts (PI570481, SC84, Sumac, white) for 8 days (total 10 days). Intracellular lipid was measured by oil red O (ORO) staining and shown as an image. The ORO was dissolved in isopropanol and absorbance was measured at 500 nm and expressed as a graph. Values are means ± SD (n = 3). Significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001. Difference is between vehicle (0 mg/mL-treated) and treatment group.