Figure 7.

Sorghum bran extracts repressed glucose uptake and insulin signaling in differentiated 3T3-L1 cells. (A) 3T3-L1 cells were treated with 0, 1.25, and 2.5 mg/mL sorghum extracts (PI570481, SC84, Sumac, white) for 10 days. Glucose uptake was measured using 2-NBDG (n = 4). (B) Western blot was performed for IRS-1 and phospho and total Akt (B–D). Values are means ± SD (n = 3). Significance is indicated as # and *. # p < 0.05, ### p < 0.001 as compared to control (undifferentiated cells). * p < 0.05, ** p < 0.01, *** p < 0.001 as compared to untreated (0 mg/mL) differentiated cells.