Type |
The soil should be natural and fertile, preferably “sandy loam” collected from fields and forests not exposed to pollutants. The soil used should be a mixture of natural and fertile soils collected from the surface layers of at least 3 diverse locations. |
Natural soil from fields and/or forests may be used as an inoculum to simulate biodegradation in a specific natural environment. |
Particle size |
Less than 2 mm |
Less than 5 mm |
Room Temperature |
20 − 28 ± 2 °C |
20 − 28 ± 2 °C but in preference 25 °C |
pH |
6–8 |
6–8 |
Moisture holding Capacity (MHC) |
80–100% |
40–60% |
Running Time |
Should not exceed 6 months |
Should not exceed 6 months but may be extended up to 24 months |
Reference Material |
Cellulose or starch |
Microcrystalline cellulose powder, ashless cellulose filters or poly(3-hydroxybutyrate) |
Validation Criteria |
The test is considered valid if after 6 months more than 70% biodegradation is achieved for the reference material and if the amount of CO2 evolved from the control reactors is within 20% of the mean at the plateau phase or at the end of the test. |
The test is considered valid if the degree of biodegradation of the reference material is more than 60% at the plateau phase or at the end of the test and if the BOD (biological oxygen demand) values of or the amount of CO2 evolved from the controls are within 20% of the mean at the plateau phase or at the end of the test. |