Table 3.
Polyphenolic compounds identified in the methanolic extracts of A. tinctoria and A. sylvestris by HPLC-ion trap MS/MS.
| tR (min) | [M-H]− | MS/MS | Plant Material/Substance | ||
|---|---|---|---|---|---|
| Aerial Parts of A. tinctoria | Aerial Parts of A. sylvestris | Roots of A. sylvestris | |||
| 0.5 | 341 | 179, 161 | Caffeic acid glucosides | Caffeic acid glucosides | Caffeic acid glucosides |
| 1.7 | 315 | 225, 153, 109 | Protocatechuic or gentisic acid glucoside | Protocatechuic or gentisic acid glucoside | nf |
| 4.3 | 325 | 163; 119 | nf | nf | Coumaric acid glucoside |
| 5.1 | 299 | 137 | 4-Hydroxybenzoic acid glucoside | 4-Hydroxybenzoic acid glucoside | nf |
| 10.3 | 339 | 281, 251, 177, 135 | Daphnin = daphnetin glucoside | nf | nf |
| 12.5 | 353 | 191, 179, 173, 135 | Neochlorogenic acid | Neochlorogenic acid | Neochlorogenic acid |
| 15.2 | 385 | 223, 179, 163 | Sinapinic acid glucoside | nf | nf |
| 15.5 | 353 | 306, 191, 135 | nf | Chlorogenic acid | nf |
| 16.5 | 639 | 463, 301, 535 | Quercetin glucoside glucuronide | nf | nf |
| 16.7 | 337 | 191, 163, 173 | nf | Coumaroylquinic acid | Coumaroylquinic acid |
| 17.3 | 625 | 463, 301 | Quercetin diglucoside |
nf | nf |
| 17.8 | 335 | 179, 135 | Caffeoylshikimic acid | nf | nf |
| 18.6 | 367 | 191, 173 | 5-Feruloylquinic acid | 5-Feruloylquinic acid | 5-Feruloylquinic acid |
| 19.8? | 479 | 317 | Myricetin glucoside | nf | nf |
| 20.9 | 625 | 301 | nf | Quercetinlucoside-glucoside | nf |
| 21.3 | 655 | 493, 331 | Patuletin diglucoside | nf | nf |
| 22.0 | 449 | 287, 151 | Eriodictyol glucoside | nf | nf |
| 22.2 | 741 | 609, 475, 343, 301 | Quercetin rutinoside pentoside | nf | nf |
| 22.6 | 477 | 301, 373 | Quercetin glucuronide | nf | nf |
| 22.8 | 463 | 301, 179, 343 | Quercetin galactoside | Quercetin galactoside | nf |
| 23.2 | 609 | 301, 343, 271 | Rutin | Rutin | Rutin, traces |
| 23.3 | 463 | 301 | Quercetin glucoside | Quercetin glucoside | Quercetin glucoside |
| 24.0 | 493 | 331, 373 | Patuletin glucoside | nf | nf |
| 24.9 | 477 | 315, 433 | Isorhamnetin glucoside | nf | nf |
| 24.9 | 505 | 463, 301, 179, 151 | Quercetin acetyl glucoside | nf | nf |
| 25.4 | 515 | 353, 191 | Dicaffeoylquinic acid 1 | Dicaffeoylquinic acid 1 | nf |
| 26.4 | 493 | 331, 287 | Patuletin-7-glucoside | nf | nf |
| 26.4 | 373 | 211, 193 | Pinosylvin glucoside | nf | nf |
| 26.7 | 477 | 315, 357, 300 | Isorhamnetin glucoside | Isorhamnetin glucoside | nf |
| 27.3 | 607 | 299, 284 | Diosmetin rutinoside = diosmin | Diosmetin rutinoside = diosmin | nf |
| 27.4 | 515 | 191, 179, 255, 299, 353 | 3,4-Dicaffeoylquinic acid-2 | 3,4-Dicaffeoylquinic acid-2 | 3,4-Caffeoylquinic acid |
| 27.7 | 489 | 285 | Kaempferol acetylglucoside | nf | nf |
| 29.1 | 529 | 353, 191 | Feruloylquinic acid glucoside | Feryloylquinic acid glucoside | nf |
| 30.4 | 535 | 331, 316 | Patuletin acetylglucoside | nf | nf |
| 31.0 | 331 | 316, 209 | Patuletin | nf | nf |
| 31.8 | 315 | 300 | Iso-rhamnetin | nf | nf |
| 32.6 | 271 | 151, 177, 119 | nf | Naringenin | Naringenin |
| 32.8 | 609 | 285 | Kaempferol rutinoside | nf | nf |
| 33.0 | 345 | 330, 315 | Spinacetin | nf | nf |
| 34.1 | 677 | 515, 353, 255, 191 | Tricaffeoylquinic acid | Tricaffeoylquinic acid | nf |
| 34.3 | 271 | 107, 119, 151 | nf | nf | Naringenin chalcone |
| 35.3 | 299 | 284 | Diosmetin | nf | nf |
| 35.7 | 315 | 300, 251 | Nepetin | nf | nf |
| 36.6 | 329 | 314, 171 | Jaceosidin | nf | nf |
| 38.1 | 359 | 344, 329 | Jaceidin | nf | nf |
| 38.3 | 593 | 447; 301 | nf | Quercetin dirhamnoside | nf |
| 38.4 | 593 | 285 | nf | Luteolin rutinoside | nf |
tR, retention time; [M-H]−, ion mass; MS/MS, mass of identified fragments; nf, not found.