Table 4.
Polyphenolic compounds identified in the methanolic extract of A. tinctoria and A. sylvestris by the UPLC–triple quadrupole MS/MS (MRM) method.
| tR (min) | Precursor Ion (m/z) [M-H]− |
Product Ion (m/z) MS/MS |
Aerial Parts of A. tinctoria | Aerial Parts of A. sylvestris | Roots of A. sylvestris |
|---|---|---|---|---|---|
| 0.46 | 191 | 85 | Quinic acid | Quinic acid | Quinic acid |
| 2.80 | 353 | 191 | Neochlorogenic acid | Neochlorogenic acid | Neochlorogenic acid |
| 3.75 | 353 | 191 | Chlorogenic acid | Chlorogenic acid | Chlorogenic acid |
| 3.98 | 179 | 107 | Caffeic acid | Caffeic acid | nf |
| 5.06 | 609 | 300 | Rutin | Rutin | nf |
| 5.15 | 593 | 285 | Luteolin 7-rutinoside | Luteolin 7-rutinoside | Luteolin 7-rutinoside |
| 5.18 | 193 | 134 | Ferulic acid | Ferulic acid | Ferulic acid |
| 5.39/5.55/5.76 | 515 | 353 | Dicaffeoylquinic acids | Dicaffeoylquinic acids | Dicaffeoylquinic acids |
| 5.22 | 463 | 301 | Hyperoside | nf | nf |
| 5.26 | 447 | 285 | Luteolin-7-glucoside | nf | nf |
| 5.28 | 463 | 301 | Isoquercitrin | Isoquercitrin | Isoquercitrin |
| 5.52 | 623 | 315 | Isorhamnetin 3-rutinoside | nf | nf |
| 5.70 | 447 | 300 | Quercitrin | Quercitrin | Quercitrin |
| 5.70 | 447 | 285 | Luteolin 4-glucoside | nf | nf |
| 6.79 | 285 | 133 | Luteolin | nf | nf |
| 6.86 | 301 | 151 | nf | Quercetin | nf |
| 7.22 | 271 | 151 | nf | Naringenin | Naringenin |
| 7.38 | 269 | 117 | Apigenin | Apigenin | Apigenin |
| 7.57 | 299 | 284 | nf | Diosmetin | Diosmetin |
| 7.60 | 315 | 300 | nf | Isorhamnetin | nf |
tR, retention time; [M-H]−, ion mass; MS/MS, mass of identified fragments; nf, not found.