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. Author manuscript; available in PMC: 2022 Apr 12.
Published in final edited form as: Cell Rep. 2022 Mar 29;38(13):110580. doi: 10.1016/j.celrep.2022.110580

Figure 5. Low concentrations of TTX reproduce the effect of heterozygous loss of Nav1.1 on PV-IN synaptic transmission.

Figure 5.

(A) Electrophysiological discharge pattern of a PV-IN from a P18 WT.PV-Cre.tdT mouse in response to a 600-ms, 800-pA current injection before (top, black) and after (middle; magenta) bath application of 10 nM TTX, with washout (bottom; gray).

(B) Repetitive stimulation of presynaptic PV-INs elicited IPSCs in postsynaptic principal cells (left) with a higher failure rate after bath application of 10 nM TTX (right). Note that PV-INs can follow 120-Hz stimulation in the presence of TTX.

(C) Prolonged synaptic latency after TTX application. Shown is a presynaptic action potential aligned to peak (top), with the onset of the postsynaptic IPSC response (bottom).

(D–F) Summary data show increased failure rate for the fifth (D) and last (E) IPSC in a train at 120 Hz and for synaptic latency (F). *p < 0.05.