| Antibodies |
|
| Rabbit anti-parvalbumin |
Swant |
Cat# PV27; RRID:AB_2631173 |
| ZNP-1 (Mouse anti-synaptotagmin 2) |
ZFIN |
Cat# ZDB-ATB-081002-25; RRID:AB_10013783 |
| Mouse anti-Nav1.1 (K74/71) |
NeuroMab |
Cat# 75–023; RRID:AB_2877321 |
| Mouse anti-Pan-Nav1 (N419/40) |
NeuroMab |
Cat# 75–405; RRID:AB_2877589 |
| Alexa Fluor 405 goat anti-rabbit |
Molecular Probes |
Cat# A31556 |
| Alexa Fluor 488 goat anti-mouse (IgG2a) |
Molecular Probes |
Cat# A21131 |
| Alexa Fluor 488 goat anti-mouse (IgG1) |
Molecular Probes |
Cat# A21121 |
|
| Bacterial and virus strains |
|
| pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST |
Addgene |
Cat# 105448-AAV9 |
| pAAV-Ef1a-DIO-hChR2(E123A)-EYFP |
Addgene |
Cat# 35507-AAV9 |
|
| Chemicals, peptides, and recombinant proteins |
|
| Tetrodotoxin citrate |
abcam |
Cat# ab120055 |
|
| Experimental models: Organisms/strains |
|
| Wild-type 129S6.SvEvTac Mice |
Taconic Biosciences |
RRID:IMSR_TAC:129sve |
| Wild-type C57BL/6J Mice |
Jackson Laboratory |
RRID:IMSR_JAX:000664 |
|
Scn1a+/− Mice |
Laboratory of Jennifer Kearney, Northwestern University |
RRID:MMRRC_037107-JAX |
| Pvalb-Cre Mice |
Jackson Laboratory |
RRID:IMSR_JAX:017320 |
| Ai14 Mice |
Jackson Laboratory |
RRID:IMSR_JAX:007914 |
|
| Oligonucleotides |
|
| Primer: Jax007914/Ai14D wt-f: AAG GGA GCT GCA GTG GAG TA |
Integrated DNA Technologies |
N/A |
| Primer: Jax007914/Ai14D wt-r: CCG AAA ATC TGT GGG AAG TC |
Integrated DNA Technologies |
N/A |
| Primer: Jax007914/Ai14D mt-f: GGC ATT AAA GCA GCG TAT CC |
Integrated DNA Technologies |
N/A |
| Primer: Jax007914/Ai14D mt-r: CTG TTC CTG TAC GGC ATG G |
Integrated DNA Technologies |
N/A |
| Primer: Jax017320/PvCre mt-f: AAA TGC TTC TGT CCG TTT GC |
Integrated DNA Technologies |
N/A |
| Primer: Jax017320/PvCre mt-r: ATG TTT AGC TGG CCC AAA TG |
Integrated DNA Technologies |
N/A |
| Primer: Jax017320/PvCre wt-f: CAG AGC AGG CAT GGT GAC TA |
Integrated DNA Technologies |
N/A |
| Primer: Jax017320/PvCre wt-r: AGT ACC AAG CAG GCA GGA GA |
Integrated DNA Technologies |
N/A |
| Primer: MMRRC_037107-JAX/Scn1atm1Kea, common-f: AGT CTG TAC CAG GCA GAA CTT G |
Integrated DNA Technologies |
N/A |
| Primer: MMRRC_037107-JAX/Scn1atm1Kea, wt-r: CCC TGA GAT GTG GGT GAA TAG |
Integrated DNA Technologies |
N/A |
| Primer: MMRRC_037107-JAX/Scn1atm1Kea, mt-r: AGA CTG CCT TGG GAA AAG CG |
Integrated DNA Technologies |
N/A |
|
| Software and algorithms |
|
| pClamp 11.0 |
Molecular Devices |
N/A |
| MATLAB |
MathWorks |
N/A |
| GraphPad Prism 9.0.1 |
GraphPad Software Inc. |
RRID: SCR_002798 https://www.graphpad.com/scientific-software/prism/
|
| Adobe Illustrator 2020 |
Adobe |
N/A |
| Fiji:ImageJ |
Open Source at https://fiji.sc
|
RRID:SCR_002285 |
| BioRender |
BioRender |
N/A |
| Source data |
This paper |
https://doi.gin.g-node.org/10.12751/g-node.4adqko
|
| Resource code |
This paper |
https://doi.org/10.5281/zenodo.6298722
|
