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. 2022 Apr 12;19:15. doi: 10.1186/s12983-022-00461-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Organization of the nervous system in the arm tip of O. brevispinum. Maximum intensity Z-projections of confocal image stacks. (A) Acetylated tubulin. Filled arrow shows the terminal aboral loop formed by the hyponeural part of the radial nerve cord, which gives off a number of short tracts (open arrows) running towards the tip. (B) Elav-positive neurons. (C) GFSKLYFamide (GFS)-positive neuronal elements. (D) Expression of the transcription factor Brn1/2/4. Note that this particular antibody, besides specifically binding to the antigen in the nucleus, also non-specifically bind to the cuticle that covers the surface of the epidermis (asterisk)White arrowheads in B–D show clusters of immunopositive neuronal cell bodies