Fig. 2.

I-GSCs are endowed in vivo with enhanced tumorigenic ability. A. GBM xenografts derived from I-GSCs display higher growth than those generated by D-GSCs sibling regardless of the transcriptional subtype, as depicted by quantitative time-course bioluminescence analysis. Data are mean ± SEM. P-values are from hierarchical linear model; n = 5 mice/group. B. Mice carrying luc-I- and D-GSCs cells are imaged from 7 days post-transplantation (DPT) to the endpoint. C. Serial immunohistochemical reconstructions confirming that I-GSCs give rise to more extended and invasive tumors than those from D-GSCs injection. Bar, 1 mm. D. Kaplan-Meier plot of survival demonstrating that animals receiving I-GSCs die much earlier than those implanted with their sibling D-GSCs. P-values are from Log-rank and Gehan-Breslow-Wilcoxon test