KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| RPL28 Polyclonal Antibody | Invitrogen | PA5-62192; RRID: AB_2646739 |
| Anti-RPL24 antibody | Abcam | ab126172; RRID: AB_11127305 |
| RSL24D1 Polyclonal antibody | Proteintech | 25190-1-AP; RRID: AB_2879950 |
| RSL24D1 Polyclonal antibody | Invitrogen | PA5-64058; RRID: AB_2646835 |
| Recombinant Anti-AFG2 antibody | Abcam | ab189519 |
| Recombinant Anti-CINP antibody | Abcam | ab183519 |
| SPATA5L1 Monoclonal Antibody (16A2) | Invitrogen | MA5-34952; RRID: AB_2848858 |
| Monoclonal ANTI-FLAG® M2 antibody | Sigma | F1804; RRID: AB_262044 |
| ANTI-FLAG® M2 Affinity Gel | Sigma | A2220; RRID: AB_10063035 |
| Anti-SNAP-tag® Antibody (Polyclonal) | NEB | P9310S; RRID: AB_10631145 |
| Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 | Sigma | MAB374; RRID: AB_2107445 |
| Chemicals, peptides, and recombinant proteins | ||
| Anisomycin | Sigma | A9789 |
| Puromycin | Sigma | P8833 |
| Cycloheximide | Sigma | C4859 |
| Sodium Arsenite | Sigma | 5092650001 |
| CX5461 | Sigma | S7400 |
| SUPERase•In™ RNase Inhibitor | Invitrogen | AM2694 |
| RNase T1 | Thermo Scientific | EN0541 |
| DSP | Thermo Scientific | 22586 |
| SNAP-Cell TMR-Star | NEB | S9105S |
| SNAP-Cell Block | NEB | S9106S |
| SNAP-Cell Oregon Green | NEB | S9104S |
| SNAP-Capture Magnetic Beads | NEB | S9145S |
| Lipofectamine 3000 | Thermo Scientific | L3000008 |
| Click-&-Go™ Click Chemistry Reaction Buffer Kit | clickchemistrytools | 1001 |
| Click-&-Go™ Cell Reaction Buffer Kit | clickchemistrytools | 1263 |
| L-Azidohomoalanine (AHA) | clickchemistrytools | 1066 |
| AZDye 488 Azide | clickchemistrytools | 1275 |
| Biotin Alkyne | clickchemistrytools | 1266 |
| NorthernMax™ Kit | Invitrogen | AM1940 |
| DMEM, high glucose, no glutamine, no methionine, no cystine | Gibco | 21013024 |
| GlutaMAX™ Supplement | Gibco | 35050061 |
| Fetal Bovine Serum, dialyzed | Gibco | A3382001 |
| L-Cysteine | Sigma | C7352 |
| HRP-Conjugated Streptavidin | Thermo Scientific | N100 |
| Deposited data | ||
| Raw CRISPR screen data | This study | GSE197277 |
| Analyzed CRISPR screen data_gene list | This study | GSE197277 |
| Analyzed CRISPR screen data_guide list | This study | GSE197277 |
| Experimental models: Cell lines | ||
| Lenti-X 293T | Takarabio | 632180 |
| 293T-RPL28-SNAP | This study | N/A |
| 293T-RPL28-SNAP-pCW57.1-C1orf109-3xFLAG-Tet-off | This study | N/A |
| HCT116-RPL28-SNAP | This study | N/A |
| H9-RPL28-SNAP | This study | N/A |
| Oligonucleotides | ||
| sgRNA for RPL28 knock-in: CTTTCTCACCTGCCTCGAC | (An and Harper, 2018) | N/A |
| See Table S1 for sgRNA for CRISPRKO | (Doench et al., 2016) | N/A |
| See Table S2 for Northern probes | (Tafforeau et al., 2013) | N/A |
| Recombinant DNA | ||
| pCW57.1-C1orf109-3xFLAG-Tet-off | This study | N/A |
| pCMV2-C1orf109 | This study | N/A |
| pCMV2-SPATA5 | This study | N/A |
| pCMV2-SPATA5-T330del | This study | N/A |
| pCMV2-SPATA5-D628G | This study | N/A |
| Software and algorithms | ||
| GraphPad Prism 8 | GraphPad | N/A |
| FlowJo v10 | FlowJo | N/A |
| MAGeCKFlute | (Wang et al., 2019) | N/A |