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. 2022 Mar 14;88(7):e02513-21. doi: 10.1128/aem.02513-21

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FIG 2 — (A) Plasmid pEA-iMGE is a pUC57 plasmid backbone which contains 4 protospacers and the kanR gene. PAM and protospacer sequences are highlighted in red and purple, respectively. (B) Plasmid pEA-CRR1Φ is a pUC18 plasmid backbone which contains the ampicillin ampR gene in addition to an artificial CRISPR array. The array is composed of the endogenous leader sequence, and the 12 spacer sequences provided, separated by CRISPR repeats. Plasmid pEA-CRR2Φ is identical to pEA-CRR1Φ except the CRISPR repeats and leader sequence were modified to match the CRR2 array of E. amylovora. (C) The CRISPR repeat sequences for CRR1 and CRR2 of E. amylovora with the 2 bp difference highlighted in blue. Created with BioRender.com.