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Curing of pEA-iMGE after secondary transformation by pUC19, pEA-CRR1Φ, and pEA-CRR2Φ. Transformations were enumerated after 30 h. Bars represent the mean and the error bars represent a 95% confidence interval of the mean among three replicates. Significance was determined using a Student’s T-test. All significant differences (**) had a P-value < 0.01.
