TABLE 1.

PCR primers used in this study

Primer	Sequence (5′–3′)	Position	Description	Reference
OP3-565F	GGGTGTAAAGGGCAGGTA	608–626a	OP3-specific 16S rRNA gene forward primer	This study
OP3-1481R	TACGACTTAGCGCCAGTC	1525–1543a	OP3-specific 16S rRNA gene reverse primer	This study
8-27F	AGAGTTTGATCCTGGCTCAG	8–27b	Bacterial universal 16S rRNA gene forward primer	71
907R	CCGTCAATTCMTTTGAGTTT	907–926b	Bacterial universal 16S rRNA gene reverse primer	72

^aOP3 LiM 16 rRNA location.

^bE. coli 16S rRNA location.