TABLE 3.
Probe name | Probe sequence (5′–3′) | Position | Target group | FA concn(%)b | Reference |
---|---|---|---|---|---|
EUB338 Ic | GCTGCCTCCCGTAGGAGT | 338–355 | Most Bacteria | 35 | 73 |
EUB338 IIc | GCAGCCACCCGTAGGTGT | 338–355 | Planctomycetales | 35 | 74 |
EUB338 IIIc | GCTGCCACCCGTAGGTGT | 338–355 | Verrucomicrobiales | 35 | 74 |
ARCH-915 | GTGCTCCCCCGCCAATTCCT | 915–934 | Archaea | 35 | 75 |
OP3-565 | TACCTGCCCTTTACACCC | 608–626d | Candidate OP3 LiM | 30 | 19 |
Alternatively, ARCH-915 was labeled with carboxyfluorescein at the bases shown in italics: GUG CTC CCC CGC CAA TTC CT.
Formamide (FA) concentration in the hybridization buffer.
Used in a mix.
OP3 LiM 16S rRNA location.