TABLE 7.
Primers used in this study
| Primer name | Sequence (5′ to 3′) | Use |
|---|---|---|
| pBbS8a_fwd | ggatccaaactcgagtaaggatctcc | pBbS8a backbone plasmid construction |
| pBbS8a_rev | atgtatatctccttcttaaaagatcttttgaattcc | pBbS8a backbone plasmid construction |
| pBbS8a-PP_2181 PP_2181_fwd | ggaattcaaaagatcttttaagaaggagatatacatatgaaagtgcacgaagaaatcgaaggcc | pBbS8a-PP_2181 construction |
| pBbS8a-PP_2181 PP_2181_rev | ggagatccttactcgagtttggatcctcagctgaacacccaaagcacgcg | pBbS8a-PP_2181 construction |
| pBbS8a-PP_0596 PP_0596_fwd | ggaattcaaaagatcttttaagaaggagatatacatatgagccgacgccccgatcc | pBbS8a-PP_0596 construction |
| pBbS8a-PP_0596 PP_0596_rev | ggagatccttactcgagtttggatcctcaacgtgtcgcagccaggc | pBbS8a-PP_0596 construction |
| BBR1k_fwd | tttaagaaggagatatacatatggcgag | BBR1k backbone plasmid construction |
| BBR1k_rev | gacgtcggaattgccagctg | BBR1k backbone plasmid construction |
| BBR1k-PP_2177p-RFP PP_2177p_fwd | gcgccccagctggcaattccgacgtcgtcgggctggaggattcagc | BBR1k-PP_2177p-RFP construction |
| BBR1k-PP_2177p-RFP PP_2177p_rev | cgctactcgccatatgtatatctccttcttaaacgtttcggactgccgtgaaatttttag | BBR1k-PP_2177p-RFP construction |
| BBR1k-PP_0596p-RFP PP_0596p_fwd | gcgccccagctggcaattccgacgtcactcgacgacgctgcgatag | BBR1k-PP_0596p-RFP construction |
| BBR1k-PP_0596p-RFP PP_0596p_rev | cgctactcgccatatgtatatctccttcttaaacggcggttcctcggaagacg | BBR1k-PP_0596p-RFP construction |
| BBR1k-PP_4578p-RFP PP_4578p_fwd | gcgccccagctggcaattccgacgtctttctgccgtcaggcggaagct | BBR1k-PP_4578p-RFP construction |
| BBR1k-PP_4578p-RFP PP_4578p_rev | accaacaagccgaggcatcctttaagaaggagatatacatatggcgagtagcg | BBR1k-PP_4578p-RFP construction |
| BBR1k-PP_2920p-RFP PP_2920p_fwd |
gcgccccagctggcaattccgacgtcaggtggatcgccgcttgc | BBR1k-PP_2920p-RFP construction |
| BBR1k-PP_2920p-RFP PP_2920p_rev |
gtgccaggagacgcggtctttaagaaggagatatacatatggcgagtagcga | BBR1k-PP_2920p-RFP construction |
| pET28_fwd | atgagatccggctgctaacaaagccc | pET28 backbone plasmid construction |
| pET28_rev | tgctagccatatggctgccgcg | pET28 backbone plasmid construction |
| pET28-PP_2180 PP_2180_fwd |
gccgcgcggcagccatatggctagcagtgaacagaattcgcagacccttgcc | pET28-PP_2180 construction |
| pET28-PP_2180 PP_2180_rev |
cgggctttgttagcagccggatctcattaccgaacagcctcataggtcaggtcc | pET28-PP_2180 construction |
| pET28-PP_5182 PP_5182_fwd |
gccgcgcggcagccatatggctagcagcgtcaacaacccgcaaaccc | pET28-PP_5182 construction |
| pET28-PP_5182 PP_5182_rev |
cgggctttgttagcagccggatctcattattgaatcgcctcaagggtcaggtcc | pET28-PP_5182 construction |
| pET28-PP_0596 PP_0596_fwd |
gccgcgcggcagccatatggctagcaacatgcccgaaactggtcctgcc | pET28-PP_0596 construction |
| pET28-PP_0596 PP_0596_rev |
cgggctttgttagcagccggatctcatcagtcgatcaggttcagggtttcgc | pET28-PP_0596 construction |
| pBADT_fwd | aggatccaaactcgagtaaggatctcc | pBADT backbone plasmid construction |
| pBADT_rev | atgtatatctccttcttaaaagatcttttgaattcc | pBADT backbone plasmid construction |
| pBADT-PP_5182 PP_5182_fwd |
tcaaaagatcttttaagaaggagatatacatatgagcgtcaacaacccg | pBADT-PP_5182 construction |
| pBADT-PP_5182 PP_5182_rev |
ggagatccttactcgagtttggatccttattgaatcgcctcaagggtcagg | pBADT-PP_5182 construction |
| pBADT-PP_0596 PP_0596_fwd |
tcaaaagatcttttaagaaggagatatacatatgaacatgcccgaaactggtcctgc | pBADT-PP_0596 construction |
| pBADT-PP_0596 PP_0596_rev |
ggagatccttactcgagtttggatcctcagtcgatcaggttcagggtttcgc | pBADT-PP_0596 construction |
| pBADT-PP_2920-2 PP_2920-2_fwd |
tcaaaagatcttttaagaaggagatatacatatgcaagcagtggatttcaactcgg | pBADT-PP_2920-2 construction |
| pBADT-PP_2920-2 PP_2920-2_rev |
ggagatccttactcgagtttggatcctcagctgaagagcctggccagg | pBADT-PP_2920-2 construction |
| pBADT-PP_4575-7 PP_4575-7_fwd |
tcaaaagatcttttaagaaggagatatacatatgcacaacgacaagggagac | pBADT-PP_4575-7 construction |
| pBADT-PP_4575-7 PP_4575-7_rev |
ggagatccttactcgagtttggatccttacgaccaacgccccagg | pBADT-PP_4575-7 construction |
| pBADT-PP_2180 PP_2180_fwd |
tcaaaagatcttttaagaaggagatatacatatgagtgaacagaattcgcagaccc | pBADT-PP_2180 construction |
| pBADT-PP_2180 PP_2180_rev |
ggagatccttactcgagtttggatccttaccgaacagcctcataggtcaggtcc | pBADT-PP_2180 construction |
| pMQ30_fwd | tctaagaaaccattattatcatgacattaacc | pMQ30 backbone plasmid construction |
| pMQ30_rev | aaaactgtattataagtaaatgcatgtatactaaac | pMQ30 backbone plasmid construction |
| PP_4575-7_up_fwd | gtttagtatacatgcatttacttataatacagttttgcgctaatccccgaagactggc | pMQ30_PP_4575-7 construction |
| PP_4575-7_up_rev | taagactgctggccctttcgcgg | pMQ30_PP_4575-7 construction |
| PP_4575-7_down_fwd | cgcccgcgaaagggccagcagtcttacatatgcctaccttaccgcgcctgc | pMQ30_PP_4575-7 construction |
| PP_4575-7_down_rev | ggttaatgtcatgataataatggtttcttagagcaactgacggtgttgtggttcgc | pMQ30_PP_4575-7 construction |
| PP_0596_up_fwd | gtttagtatacatgcatttacttataatacagttttcaccaggttggggcgccg | pMQ30_PP_0596 construction |
| PP_0596_up_rev | atcggcggttcctcggaagacg | pMQ30_PP_0596 construction |
| PP_0596_down_fwd | taatcgtcttccgaggaaccgccgatgtgatctttccgatattgatgccaggcg | pMQ30_PP_0596 construction |
| PP_0596_down_rev | ggttaatgtcatgataataatggtttcttagacagcgccttgatctgtggctcc | pMQ30_PP_0596 construction |
| PP_2920-2_up_fwd | gtttagtatacatgcatttacttataatacagttttcatggaagtaattgcgcaccg | pMQ30_PP_2920-2 construction |
| PP_2920-2_up_rev | catgaccgcgtctcctggc | pMQ30_PP_2920-2 construction |
| PP_2920-2_down_fwd | cacaagtgccaggagacgcggtcatgtgagcatcatgttttcgctgtagccg | pMQ30_PP_2920-2 construction |
| PP_2920-2_down_rev | ggttaatgtcatgataataatggtttcttagacccgcccaggccgagctg | pMQ30_PP_2920-2 construction |
| PP_5182_up_fwd | gtttagtatacatgcatttacttataatacagttttcgcccccgagatggagttctacc | pMQ30_PP_5182 construction |
| PP_5182_up_rev | ggcggtccaataacggagaagcacat | pMQ30_PP_5182 construction |
| PP_5182_down_fwd | ggcggtccaataacggagaagcacatgtaagcgtttgctaggctagcgt | pMQ30_PP_5182 construction |
| PP_5182_down_rev | ccggtactttctacgacatggtcgccatctaagaaaccattattatcatgacattaacc | pMQ30_PP_5182 construction |
| PP_2180_up_fwd | gtttagtatacatgcatttacttataatacagttttaaacaaataccaggagcaactggacttcatgca | pMQ30_PP_2180 construction |
| PP_2180_up_rev | acagctaaccaggccctctgctgttacattgacttcactccatcaagttgcgcagc | pMQ30_PP_2180 construction |
| PP_2180_down_fwd | taacagcagagggcctgg | pMQ30_PP_2180 construction |
| PP_2180_down_rev | ggttaatgtcatgataataatggtttcttagaccaccgccgggtccatctc | pMQ30_PP_2180 construction |
| PP_0596_KOCON_fwd | ggatgtcgaaatcgctgact | Knockout confirmation for ΔPP_0596 |
| PP_0596_KOCON_rev | gcgaaatcttggcttcgttc | Knockout confirmation for ΔPP_0596 |
| PP_2180_KOCON_fwd | cgagttccaggtcatggataa | Knockout confirmation for ΔPP_2180 |
| PP_2180_KOCON_rev | gttcgacctgggacagaaa | Knockout confirmation for ΔPP_2180 |
| PP_5182_KOCON_fwd | gcagtacctgggcaagaaa | Knockout confirmation for ΔPP_5182 |
| PP_5182_KOCON_rev | cttgatccctgtctgcttctc | Knockout confirmation for ΔPP_5182 |
| PP_4575-7_KOCON_fwd | tatgtgtggatccagcgtttc | Knockout confirmation for ΔPP_4575-7 |
| PP_4575-7_KOCON_rev | ttcttcgcctcgggtatct | Knockout confirmation for ΔPP_4575-7 |
| PP_2920-2_KOCON_fwd | gaactttggcgcacctacta | Knockout confirmation for ΔPP_2920-2 |
| PP_2920-2_KOCON_rev | ggagatgttcaggtggatcg | Knockout confirmation for ΔPP_2920-2 |
| pMQ30_seq_fwd | ttgtctcatgagcggatacatatt | Forward sequencing of inserts in pMQ30 plasmids |
| pMQ30_seq_rev | tcaccgtcatcaccgaaac | Reverse sequencing of inserts in pMQ30 plasmids |
| RFPseq_up_rev | ggaaggacagtttcaggtagt | Sequencing region upstream of RFP in reporter plasmids |
| pBbS8a_seq_rev | ccttactcgagtttggat | Reverse sequencing of inserts in pBbS8a plasmids |
| pBADT_seq_fwd | atgccatagcatttttatcc | Forward sequencing of inserts in pBADT and pBbS8a plasmids |
| pBADT_seq_rev | gccctaggtataaacgcagaaa | Reverse sequencing of inserts in pBADT plasmids |
| pET28a_seq_fwd | taatacgactcactataggg | Forward sequencing of inserts in pET28a plasmids |
| pET28a_seq_rev | gctagttattgctcagcgg | Reverse sequencing of inserts in pET28a plasmids |