Fig. 3.

Presence and absence of PNX and nesfatin precursors and SREB receptors in the investigated species. Phylogenomic tree of the investigated species, annotated with the presence/absence of PNX precursor, NUCB and its nesfatin-1 peptide, and SREB receptors. The PNX neuropeptide precursor is conserved across metazoans and in the choanoflagellate S. rosetta, whereas GPR173 (proposed as a potential receptor for this peptide) is only present in Bilateria. The NUCB precursor gene is conserved across metazoans and present in choanoflagellates and Tunicaraptor, whereas the nesfatin-1 peptide is only encoded in metazoans. A question mark indicates that the NUCB sequence was only partially recovered with the N-terminal part that encodes the nesfatin-1 peptide missing in the transcriptome. An unfilled box indicates that the corresponding gene was not identified. Dashed lines in main bilaterian branches indicate generally contradicting results in different phylogenomic analyses.