Table 4.
Treelet Cluster Stability Assessment.
| Adaptive Immunity | Innate Immunity | Soluble Molecules | Allergy Immunity | Chemokines | |||||
|---|---|---|---|---|---|---|---|---|---|
| TC1 | % | TC2 | % | TC3 | % | TC4 | % | TC5 | % |
| IL-2 | 83.0 | IL-1β | 85.2 | sTNFRI | 94.0 | IL-5 | 96.1 | ITAC | 80.6 |
| Fractalkine | 83.0 | TNFα | 85.2 | sIL-2Rα | 94.0 | IL-13 | 96.1 | RANTES | 80.6 |
| IL-12p70 | 83.0 | IL-6 | 85.2 | sTNFRII | 94.0 | ||||
| IL-7 | 83.0 | MIP1β | 78.6 | sVCAM-1 | 41.7 | ||||
| IL-21 | 83.0 | MIP3α | 77.3 | ||||||
| IFNγ | 70.2 | ||||||||
| IL-23 | 70.2 | ||||||||
| IL-17A | 63.7 | ||||||||
| IL-10 | 15.4 | GM-CSF | 16.0 | - | - | IFN-γ | 10.2 | IL-8 | 7.2 |
| IL-4 | 13.6 | IL-8 | 5.7 | - | - | IL-17A | 10.2 | - | - |
| - | - | - | - | - | - | IL-23 | 10.2 | - | - |
A 1000-fold iterative bootstrap of treelet transform performed with 5 clusters and a cut-level of 16 yielded sufficiently stable cluster formation. That is all markers contained in the final model appeared at least 60% of all bootstrapping iterations, with the exception of sVCAM-1 (41.7%) for which inclusion was justified based on biological relevance to other cluster variables. Markers below the solid line were excluded from the final treelet model due to low clustering percentages. Event rate for which an inflammatory marker was contained by a particular cluster is denoted by the clustering percentage (%).