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. 2022 Feb 11;82(7):1234–1250. doi: 10.1158/0008-5472.CAN-21-1168

Figure 2.

Figure 2. A MYC-TFEB circuit is manifest during B-cell development and mitogenic signaling. A, Log2 expression levels of Tfeb, Tfeb target genes, Myc, Mycn, and CD19 transcripts at different stages of mouse B-cell development (Immgen Dataset). B, Gene expression profile of LPS-stimulated naïve mouse splenic B cells (GSE37222). Log2 gene expression of Tfeb target genes was plotted in a dot plot ordered on the basis of expression. Each dot represents one sample, and dot size corresponds to its statistical significance as shown. C, qRT-PCR analyses of expression of Myc, Odc, Tfeb, and the indicated TFEB target genes in naïve mouse splenic B220+ B cells (unstimulated) or following stimulation of these cells with LPS for 4 hours (+ LPS), or naïve mouse splenic B220+ B cells pretreated with the Myc inhibitor Myci361 for 2 hours, followed by stimulation of these cells with LPS for 4 hours (+ LPS) versus those not treated with LPS (unstimulated). D, qRT-PCR analyses of Myc, Odc, Tfeb, and TFEB target genes in pre-B cells that were deprived of IL7 for 18 hours (unstimulated) and then treated with IL7 (+ IL7) for 6 hours in the absence or presence of the Myc inhibitor Myci361. Statistical analysis: C and D, Student t tests were performed. Data are represented as mean ± SD (n = 3). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.

A MYC-TFEB circuit is manifest during B-cell development and mitogenic signaling. A, Log2 expression levels of Tfeb, Tfeb target genes, Myc, Mycn, and CD19 transcripts at different stages of mouse B-cell development (Immgen Dataset). B, Gene expression profile of LPS-stimulated naïve mouse splenic B cells (GSE37222). Log2 gene expression of Tfeb target genes was plotted in a dot plot ordered on the basis of expression. Each dot represents one sample, and dot size corresponds to its statistical significance as shown. C, qRT-PCR analyses of expression of Myc, Odc, Tfeb, and the indicated TFEB target genes in naïve mouse splenic B220+ B cells (unstimulated) or following stimulation of these cells with LPS for 4 hours (+ LPS), or naïve mouse splenic B220+ B cells pretreated with the Myc inhibitor Myci361 for 2 hours, followed by stimulation of these cells with LPS for 4 hours (+ LPS) versus those not treated with LPS (unstimulated). D, qRT-PCR analyses of Myc, Odc, Tfeb, and TFEB target genes in pre-B cells that were deprived of IL7 for 18 hours (unstimulated) and then treated with IL7 (+ IL7) for 6 hours in the absence or presence of the Myc inhibitor Myci361. Statistical analysis: C and D, Student t tests were performed. Data are represented as mean ± SD (n = 3). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.