Table 4.
Partial validation of hydroxycitric acid (HCA) in GD18 Hsd:Sprague Dawley® SD® rat fetal homogenate
| Matrix Concentration Range (ng/g) | 60-3000 |
|---|---|
| Coefficient of Determination (r2) | > 0.993 |
| LOD a (ng/g) | 7.77 |
| LOQ (ng/g) | 60.0 |
| Accuracy (RE b%) | |
| Intra-Day c | ≤ ±12.3 |
| Intra-Day d | ≤ ±11.9 |
| Precision RSDe (%) | |
| Intra-Day c | ≤ 14.5 |
| Intra-Day d | ≤ 5.5 |
| Method Verification | |
| Accuracy (REb %) | 2.6 |
| Precision (RSDe %) | 2.8 |
a
LOD = Limit of Detection; 3 x Standard Deviation for 6 lower limit of quantitation (LOQ) replicates
b
%RE = percent relative error
c
Estimated based on fetal homogenate calibration standards
d
Estimated based on fetal homogenate on low-, mid-, and high-level (90, 900, and 2260 ng/g, respectively) Quality Control (QC) samples
e
%RSD = percent relative deviation.