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(a) AetF reaction and analysis by LC-MS. Chromatograms shown are extracted ion chromatograms (EICs) for 4 (m/z 205.0972), 5 (m/z 283.0077), and 6 (m/z 362.9162) in positive ionization mode. (b) Sequence similarity network of representative, functionally characterized FMOs and the flavin-dependent halogenases from the AETX (1) biosynthetic gene cluster. See Figure S8 for a comprehensive network analysis with 3347 flavin-dependent halogenase sequences.
