| Antibodies |
| Cleaved Caspase-3 |
Cell Signaling |
Cat#9661, RRID:AB_2341188 |
| Ki67 |
Abcam |
Cat#ab15580, RRID:AB_443209 |
| Anti-Histone H3(acetyl K37) |
Abcam |
Cat#ab4729, RRID:AB_2118291 |
| Anti-Histone H3 |
Abcam |
Cat#ab1791, RRID:AB_302613 |
| Anti-Histone H4 |
Abcam |
Cat#ab10158, RRID:AB_296888 |
| Histone H3ac (pan-acetyl) |
Active Motif |
Cat#39040, RRID:AB_2687871 |
| Histone H4ac (pan-acetyl) |
Active Motif |
Cat#39026, RRID:AB_2687872 |
| Acetyl-lysine |
Cell Signaling |
Cat#9441, RRID:AB_331805 |
| α-Smooth muscle actin |
Santa Cruz |
Cat# sc-32251, RRID:AB_262054 |
| 4 Hydroxynonenal |
Abcam |
Cat#ab46545, RRID:AB_722490 |
| β-actn |
Cell Signaling |
Cat#4970, RRID:AB_2223172 |
| Biotinylated Goat Anti-Rabbit IgG |
Vector |
Cat#BA-1000, RRID:AB_2313606 |
| Cytokine multiplex antibodies |
R&D |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| [U-13C] glucose |
Cambridge isotope laboratory |
Cat#CLM-1396 |
| [U-13C] lactate |
Cambridge isotope laboratory |
Cat#CLM-1579 |
| [U-13C] oleate |
Cambridge isotope laboratory |
Cat#CLM-8763 |
| [U-13C] palmitate |
Cambridge isotope laboratory |
Cat#CLM-6059 |
| [U-13C] 3-HB |
Cambridge isotope laboratory |
Cat#CLM-3853 |
| [U-13C] glutamine |
Cambridge isotope laboratory |
Cat#CLM-1822 |
| [U-13C] leucine |
Cambridge isotope laboratory |
Cat#CLM-2262 |
| [U-13C] valine |
Cambridge isotope laboratory |
Cat#CLM-2249 |
| [U-13C] isoleucine |
Cambridge isotope laboratory |
Cat#CLM-2248 |
| [U-13C] acetate |
Cambridge isotope laboratory |
Cat#CLM-440 |
| [2-2H] lactate |
CDN Isotopes |
Cat#D-3837 |
| [3,4,4,4-2H] 3-HB |
Cayman Chemicals |
Cat#14158 |
| Abraxane |
Abraxis Bioscience |
Cat#NDC code: 68817-134-50 |
| Gemcitabine |
Hospira |
Cat#NDC code: 0409-0181-01 |
| Cisplatin |
Sigma |
Cat#PHR1624 |
| 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide |
Sigma |
Cat#03449 |
| 3-Nitrophenylhydrazine |
Sigma |
Cat#N21804
|
| pyridine |
Sigma |
Cat#270970 |
| DMEM |
Corning |
Cat# 10-017-CV |
| Matrigel |
Corning |
Cat# 354234 |
| Lactate dehydrogenase |
Sigma |
Cat#59747 |
| hydroxybutyrate dehydrogenase |
Sigma |
Cat#10127833001 |
| TRIzol |
Invitrogen |
Cat#15596026 |
| NAD |
Sigma |
Cat#N0632 |
| RIPA buffer (containing |
Millipore |
Cat#20-188 |
| proteinase inhibitor |
Roche |
Cat#4693159001 |
| goat serum |
Fisher Scientific |
Cat#16210064 |
| horseradish peroxidase streptavidin |
Vector Laboratories |
Cat#SA-5704-100, |
| DAB |
Agilent/Dako |
Cat#K346811-2 |
| hematoxylin |
Sigma |
Cat#GHS216 |
| Cytoseal 60 mounting medium |
Thermo Scientific |
Cat#23-244256 |
| Sirus Red |
Sigma |
Cat#365548 |
| SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) |
Cell Signaling |
Cat#8114, RRID:AB_10544930 |
| Immunohistochemistry Application Solutions Kit for rabbit |
Cell Signaling |
Cat#13079 |
| Critical commercial assays |
| insulin |
crystal chem |
Cat#90080 |
| c-peptide |
crystal chem |
Cat#90050 |
| IGF1 |
R&D systems |
Cat#MG100 |
| 4-HNE |
Cell Biolabs |
Cat#STA-838 |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6 |
Charles River Laboratories |
Cat# 027; RRID:IMSR_CRL:027 |
| Mouse: NSG |
Jackson Laboratory |
Cat# 005557; RRID:IMSR_JAX: 005557 |
| Mouse: KPC (KrasLSL-G12D/+Trp53R172H/+Pdx-1-Cre) mice |
From Haiyonghan’s lab |
N/A |
| Mouse: KrasLSL-G12D/+Trp53−/− Pdx-1-Cre mice |
From Shawn Davidson’s lab |
N/A |
| Software and Algorithms |
| El-Maven |
Elucidata |
https://resources.elucidata.io/elmaven
|
| GraphPad Prism 7 |
GraphPad Software |
https://www.graphpad.com/scientificsoftware/prism/
|
| Isocope |
In house developed |
|
| Image J |
Image J |
https://imagej.nih.gov/ij/
|
| Deposited Data |
Mendeley data |
https://data.mendeley.com/datasets/chm3xr2j7v/1
|
| Others: |
| Ketogenic diet |
Bio-serv |
Cat#S3666 |
| Control diet |
Lab diet |
Cat#PicoLab 5053 |
| LC-MS Q-Exactive plus |
Thermofisher |
Cat# IQLAAEGAAPFALGMBDK |
| Biotek Synergy HT |
Biotek |
N/A |
| XBridge BEH Amide XP column |
Waters |
Cat# 186006725 |
| Acquity UPLC BEH C18 column |
Waters |
Cat# 186002350 |
| Mouse Button |
Instech |
Cat# VABM1B/25 |
| Mouse Jugular Vein Catheter |
Instech |
Cat# C20PU-MJV1301 |
| Mouse Double Catheter Button |
Instech |
Cat# VABM2B/25R25 |
| Mouse Carotid Artery Catheter |
Instech |
Cat# C10PU-MCA1459 |
| Surgery tools |
Fine Science Tools |
N/A |
| Cyromill |
Retsch |
Cat# 20.749.0001 |
| Beads |
Inframat |
Cat# 4039GM-S005 |
| microvette CB300 Z |
Sarstedt |
Cat# 16.440 |
| SolariX XR MS |
Bruker |
https://www.bruker.com/products/mass-spectrometry-and-separations/mrms/solarix.html
|
| Just infusion Syringe Pump |
Just infusion |
Cat# NE-300 |
| Visualsonics Vevo 770 system |
Fujifilm VisualSonics |
N/A |
| cryomicrotome |
Leica |
Cat#CM3050S |
| HTX TM-Sprayer |
HTX Technologies |
N/A |
| ChemiDoc MP system |
Bio-Rad |
N/A |
| Nikon Eclipse 80i microscope |
Nikon |
N/A |
