Fig 1. Interaction of CD47 with its ligand SIRPα is necessary for Ag-induced CD4^{+ T} cell expansion in vivo.

(A) T cell adoptive transfer scheme. (B) Proliferation of adoptively transferred violet dye labeled CD4⁺ OTII T cells recovered from WT, Cd47KO or SKI recipient mice. Each peak indicates one cell division. (C-F) OTI cells recovered after 72 h or 6 days, and division and proliferation indices of transferred OTII T cells post OVA immunization. Means ± SEM, 4–6 animals per group, and representative of 2 independent experiments. * p<0.05, ***p<0.001 compared to WT by one-way ANOVA with Tukey test. (G) Representative CD62L and CD44 surface expression on gated double positive CD4⁺ WT, Cd47KO and SKI OTII T cells recovered from spleens of SKI, Cd47KO or WT mice. The number above the open rectangle is the average percentage of CD44^hi effector T cells. Data are means ± SEM from 3–4 mice per group. * p< 0.05 compared to WT-OVA by one-way ANOVA with Tukey test.