Table 1. High-confidence validated PPT1 substrates.

Protein name (UniProt ID)	Gene name	Description	Palmitome ratio	Palmitome p-value	Proteome ratio	Proteome p-value	CRAPome (%)	Kang and colleagues	Swiss Palm (%)	CSS Palm
AT1A1_MOUSE	Atp1a1	Sodium/potassium-transporting ATPase subunit alpha-1	1.831	0.007	0.960	0.318	10.949		5.882	X
AT1A2_MOUSE	Atp1a2	Sodium/potassium-transporting ATPase subunit alpha-2	1.744	0.003	1.068	0.498	22.871		5.882
AT1A3_MOUSE	Atp1a3	Sodium/potassium-transporting ATPase subunit alpha-3	1.746	0.030	1.042	0.695	24.574		5.882
AT1B1_MOUSE	Atp1b1	Sodium/potassium-transporting ATPase subunit beta-1	2.180	0.019	0.982	0.536	0.243	X	5.882
AT1B2_MOUSE	Atp1b2	Sodium/potassium-transporting ATPase subunit beta-2	6.852	0.018	1.043	0.471	0.000	X	5.882	X
ATPO_MOUSE	Atp5po	ATP synthase subunit O, mitochondrial	6.138	0.044	1.060	0.277	0.000	X	5.882
BASI_MOUSE	Bsg	Basigin	2.695	0.016	0.927	0.101	9.732	X	5.882	X
CADM2_MOUSE	Cadm2	Cell adhesion molecule 2	1.975	0.004	0.988	0.559	0.000		11.76	X
CD81_MOUSE	Cd81	CD81 antigen	2.554	0.042	1.035	0.940	0.243		29.41
CISD1_MOUSE	Cisd1	CDGSH iron-sulfur domain-containing protein 1	1.920	0.050	0.992	0.635	1.703		35.29
DYL2_MOUSE	Dynll2	Dynein light chain 2, cytoplasmic	4.192	0.027	0.996	0.808	9.246		0	X
GBB2_MOUSE	Gnb2	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	2.963	0.003	0.985	0.636	15.329		2.92	X
GPM6A_MOUSE	Gpm6a	Neuronal membrane glycoprotein M6-a	5.625	0.007	1.043	0.658	0.243	X	5.882	X
GRIA1_MOUSE	Gria1	Glutamate receptor 1	1.866	0.046	0.992	0.635	0.000	X	5.882	X
KCRB_MOUSE	Ckb	Creatine kinase B-type	1.815	0.029	0.959	0.242	39.659		11.76
LDHB_MOUSE	Ldhb	L-lactate dehydrogenase B chain	1.928	0.050	1.006	0.939	39.903	X	11.76
LGI1_MOUSE	Lgi1	Leucine-rich glioma-inactivated protein 1	2.047	0.013	1.014	0.883	0.000	X	11.76	X
NDKA_MOUSE	Nme1	Nucleoside diphosphate kinase A	5.185	0.020	1.058	0.169	29.927		29.41
NDUS1_MOUSE	Ndufs1	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial	1.859	0.005	0.967	0.283	4.623		29.41	X
NFASC_MOUSE	Nfasc	Neurofascin	1.524	0.033	1.028	0.745	0.000	X	35.29	X
NRCAM_MOUSE	Nrcam	Neuronal cell adhesion molecule	1.714	0.011	1.148	0.314	0.000		35.29	X
NTRI_MOUSE	Ntm	Neurotrimin	4.060	0.033	0.988	0.574	0.000	X	47.06	X
STXB1_MOUSE	Stxbp1	Syntaxin-binding protein 1	1.752	0.036	1.058	0.358	0.487		7.056	X
THY1_MOUSE	Thy1	Thy-1 membrane glycoprotein	3.357	0.022	1.069	0.541	0.487		17.65
VDAC2_MOUSE	Vdac2	Voltage-dependent anion-selective channel protein 2	2.929	0.029	0.946	0.058	14.112	X	35.29	X
VISL1_MOUSE	Vsnl1	Visinin-like protein 1	2.451	0.040	0.912	0.039	0.243	X	41.18	X

X = validation of given dataset; blank cells or a score of zero indicate lack of identification in that dataset [12,31,39,97]. SwissPalm score indicates percentage of palmitoylation experiments in which the protein has been identified.